Current 
              Study Areas 
            Upper 
              Mississippi National Wildlife and Fish Refuge 
            
               The 
                Upper Mississippi National Wildlife and Fish Refuge (Refuge) includes 
                most of the floodplain along more than 400 km of the Mississippi 
                River that forms the borders of Minnesota, Wisconsin, Illinois, 
                and Iowa.  More people visit this Refuge each year than any 
                other Refuge in the United States. 
             
            
            
              -  
                
The physical nature of the floodplain of the 
                  Mississippi River in the Refuge is defined to a large extent 
                  by the system of locks and dams that have created areas referred 
                  to as pools along the river.  The Refuge is contained mostly 
                  within Pools 4 - 14 (Alma, Wisconsin, to Bettendorf, Iowa). 
               
             
            
               
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                U.S. Army Corps of Engineers' map of lock 
                    and dams  | 
               
               
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                - Populations of amphibians that live in the floodplains of 
                  large rivers have been studied less than those living in many 
                  other types of wetlands.
 
               
              
                - Populations in the Refuge face several threats, including 
                  nearby habitat loss and fragmentation, exposure to contaminants, 
                  and changes in the frequencies of floods in association with 
                  climate.
 
               
              
             
             
             
            
             St. 
              Croix National Scenic Riverway 
             
              
                - This St. Croix National Scenic Riverway (Riverway) includes 
                  approximately 400 km of riverine habitat along the Namekagon 
                  and St. Croix Rivers; the St. Croix River forms a substantial 
                  portion of the border between Wisconsin and Minnesota.
 
                   
                 
                - As with the Refuge, the Riverway provides a long, narrow strip 
                  of protected habitat in a largely unprotected landscape, where 
                  land uses, such as urbanization, logging, and agriculture can 
                  reduce the distribution and abundance of amphibians. 
 
                   
                 
               
             
            
            Voyageurs 
              National Park 
             
              
                - Voyageurs National Park is in northern Minnesota along the 
                  border with Canada.
 
                 
               
             
            
              - Voyageurs contains a landscape different from 
  
             
             
               those within the Refuge 
                and Riverway.  Three principal differences are the primarily 
                lentic (still water), rather than lotic (moving water), aquatic 
                systems, the land use outside of the park includes less agriculture 
                and more logging, and the shape of the park, which is not long 
                and linear like the Refuge and Riverway. 
                 
               
             
            
            Other 
              mid-level and apex sites 
            Port 
              Louisa National Wildlife Refuge Complex 
            
              - Port Louisa National Wildlife Refuge consists of four subunits 
                starting south of the Upper Mississippi River National Wildlife 
                and Fish Refuge and extending southward along the Mississippi 
                River between Illinois and Iowa.
 
                 
               
             
             
              
                - We will be conducting surveys in portions of this Refuge, 
                  with a special emphasis on Blanchard’s cricket frogs. 
                    We might include breeding sites of Blanchard’s 
                  cricket frogs located in this Refuge in the set of apex sites 
                  where we will study this species in more detail. 
 
               
             
            Neal 
              Smith National Wildlife Refuge 
             
              
                - Neal Smith National Wildlife Refuge is just southeast of Prairie 
                  City, Iowa, and east of Des Moines, Iowa.
 
                   
                 
                - This Refuge is unique because it is the site of one of the 
                  largest prairie restoration efforts in the country.  Based 
                  upon preliminary surveys we conducted in 2003, several amphibian 
                  species live there, including Blanchard’s cricket frogs. 
                    We expect to include breeding sites of Blanchard’s 
                  cricket frogs from the Neal Smith Refuge in our set of apex 
                  sites for studying this species.  We also will monitor 
                  other species and include this Refuge in larger studies of the 
                  effects of habitat fragmentation and loss on populations of 
                  amphibians. This latter effort will be in collaboration with 
                  researchers from Iowa State University. 
 
               
             
            Other 
              areas in Minnesota, Iowa, Illinois, and Wisconsin 
             
              
                -  
                  
We are surveying historical and potential 
                    breeding sites for Blanchard’s cricket frogs in Minnesota, 
                    Iowa, Illinois, and Wisconsin to document the current distribution 
                    of this species in portions of its northern range.  We 
                    intend to study this species more intensively at several breeding 
                    sites. 
                 
               
             
            Other 
              potential study areas in the Midwest Region 
             
              
                - All lands managed by the Department of the Interior in the 
                  Midwest Region of ARMI are potential study areas for 
                  us eventually. This also is true for areas managed by other 
                  Federal and State agencies.  As we develop expected rotations 
                  of monitoring sites over time, we will conduct surveys and monitoring 
                  in other areas.
 
               
             
            Studies 
              of Exposure to Pesticides 
             
              
                - We sampled water from 100 breeding sites for the presence 
                  of triazines during 2003. These sites were in the Upper Mississippi 
                  National Wildlife and Fish Refuge, the St. Croix National Scenic 
                  Riverway, and Voyageurs National Park.  We also collected 
                  metamorphosed northern leopard frogs (Rana pipiens) 
                  from seven of those breeding sites to analyze for any gonadal 
                  abnormalities and to determine if frequencies of gonadal abnormalities 
                  were associated with levels of triazines.  We are working 
                  to analyze those gonads, collect and analyze more water samples 
                  for triazines, and to collect and to analyze gonads from more 
                  animals during 2004.
 
                 
               
             
            Studies 
              of Disease and Deformities; Collaborations with Dr. David Green 
             
              
                - Dr. David Green is a veterinary pathologist and an expert 
                  on diseases and deformities found in amphibians and other wildlife.
 
                 
               
              
                - As a USGS researcher at the National 
                  Wildlife Health Center, Dr. Green is working with ARMI scientists 
                  to characterize the distributions of disease and deformities 
                  throughout the country. We send animals from our study areas 
                  for analyses of such afflictions to Dr Green.
 
               
             
            Studies 
              of Water Chemistry with staff from USGS's Water Resources 
             
              
                - Staff from USGS's Water Resources are integrated into ARMI.
 
                   
                 
                - We work with Perry Jones of the USGS  in St. Paul, Minnesota, to 
                  collect water samples from our study sites and have them analyzed 
                  for various chemical constituents.  We are collaborating 
                  with Perry Jones on our studies of triazines and other compounds 
                  at breeding sites.
 
               
             
            Cooperative 
              Efforts on Effects of Habitat Loss and Fragmentation and Climate 
              with staff from the USGS's Earth Resources Observation Systems (EROS) Data Center 
             
              
                - Staff from EROS are integrated into ARMI.
 
                   
                 
                - We are working with Drs. Alisa Gallant and Robert Klaver from 
                   EROS  to evaluate relations between habitat loss 
                  and fragmentation and the vulnerability of populations to decline 
                  and relations between climatic patterns over several decades 
                  and declines of Blanchard’s cricket frogs. 
 
               
             
            Collaborative 
              Efforts with Outside Researchers 
            Iowa 
              State University 
             
              
                - The natural habitat of Iowa has been altered greatly for agriculture, 
                  including the loss of approximately 98% of the state’s 
                  wetlands.
 
                   
                 
                - We are working with faculty and students from Iowa State University 
                  to study effects of habitat loss and fragmentation on populations 
                  of amphibians in Iowa.
 
               
             
            Evaluations 
              of Statuses of Populations Based Upon Results from non-USGS Studies 
             
              
                - We are working to collect and evaluate as much data as possible 
                  regarding the status of populations across the 13 states of 
                  our region. Researchers associated with state agencies, other 
                  Federal agencies, museums, and academic institutions produced 
                  these data.
 
                 
               
             
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