Upper Midwest Environmental Sciences Center
Determination of Antimycin-A in Water by Liquid Chromatographic/Mass Spectrometry: Single-Laboratory Validation
Bernardy, J.A., T.D. Hubert, J.M. Ogorek, L.J. Schmidt. 2013. Determination of Antimycin-A in Water by Liquid Chromatographic/Mass Spectrometry: Single-Laboratory Validation. Journal of AOAC International. v 96, i 2 p 413-329. DOI: http://dx.doi.org/10.5740/jaoacint.12-286
An LC/MS method was developed and validated for the quantitative determination and confirmation of antimycin-A (ANT-A) in water from lakes or streams. Three different water sample volumes (25, 50, and 250 mL) were evaluated. ANT-A was stabilized in the field by immediately extracting it from water into anhydrous acetone using SPE. The stabilized concentrated samples were then transported to a laboratory and analyzed by LC/MS using negative electrospray ionization. The method was determined to have adequate accuracy (78 to 113% recovery), precision (0.77 to 7.5% RSD with samples ≥500 ng/L and 4.8 to 17% RSD with samples ≤100 ng/L), linearity, and robustness over an LOQ range from 8 to 51 600 ng/L.