Upper Midwest Environmental Sciences Center
Efficacy of formalin and hydrogen peroxide to increase survival of channel catfish infected with saprolegniasis
Rach, J. J., T. Schreier, M. Gaikowski, and S. Schleis, 2005, Efficacy of formalin and hydrogen peroxide to increase survival of channel catfish infected with saprolegniasis: North American Journal of Aquaculture, v. 67, p. 312-318.
The efficacy of formalin and hydrogen peroxide to increase survival associated with external saprolegniasis on channel catfish (Ictalurus punctatus) fingerlings was evaluated in four laboratory trials. Fungal epizootics were initiated according to procedures developed in a fungal-disease model. Fish were abraded with a dremel tool then placed in an aerated static infection tank containing fungal-infected hemp seeds held in teaballs that were suspended in 100 L of water for 24 (trial 1-3 ) or 6 h (trial 4). In the formalin trials, treatment regimens of 0, 82, and 166 mg/L (trial 1) or 0 and 83 mg/L (trial 2) were administered to channel catfish for 60 min once every-other-day for a total of three treatments (trial 1) or once daily for a total of seven treatments (trial 2). In the hydrogen peroxide trials, treatment regimens of 0, 75, and 102 mg/L (trial 3) or 0 and 75 mg/L (trial 4) were administered to channel catfish for 60 min once every-other-day for a total of three treatments. Each treatment regimen was tested in triplicate and each replicate contained 10 fish. Formalin exposures of 83 (seven treatments; 33% survival) and 166 mg/L (three treatments; 63% survival) for 60 min significantly (P ≤ 0.01) increased channel catfish survival in comparison to controls (3% or 13% survival, respectively). In trial 4, hydrogen peroxide exposure of 75 mg/L (63% survival) for 60 min significantly (P ≤ 0.01) increased channel catfish survival in comparison to the untreated controls (13% survival). Formalin and hydrogen peroxide treatments were effective in increasing survival in channel catfish infected with fungus
formalin, hydrogen peroxide, channel catfish Ictalurus punctatus, fungal-disease