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Upper Midwest Environmental Sciences Center

          MACROINVERTEBRATE LIABILITY DISCLAIMER AND FORMAT DESCRIPTION             

                              Disclaimer                                       

These data have been generated by State agencies under contract with the       
Department of the Interior and the Upper Midwest Environmental Sciences Center 
(UMESC). Although these data have been processed successfully on a computer    
system at the UMESC, no warranty, expressed or implied, is made regarding the  
accuracy or utility of the data on any other system or for general or          
scientific purposes, nor shall the act of distribution constitute any such     
warranty. Error correction is a continuing process, and UMESC corrects data    
data errors routinely when a correct value can be determined. UMESC does not   
delete questionable data when the correct value cannot be determined, but      
attempts to mark such data with an appropriate flag. Users are strongly        
cautioned to check the accuracy of any data obtained and to report suspected   
values to UMESC.  Methods, purposes, and conditions can vary dramatically      
among data-collecting efforts and these data should not be combined with other 
datasets from the UMESC or other sources unless the user fully understands the 
procedures and purposes used to create each set of data and can verify that    
data are comparable across sources.                                            
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                                Crediting Language

These data are a product of the U.S. Army Corps of Engineers' Upper Mississippi
River Restoration Program (UMRR) Long Term Resource Monitoring (LTRM) element
implemented by the U.S. Geological Survey in collaboration with the five
Upper Mississippi River System (UMRS) states of Illinois, Iowa, Minnesota,
Missouri, and Wisconsin. The U.S. Army Corps of Engineers (Corps)
provides guidance and has overall program responsibility.                       

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                   MACROINVERTEBRATE TREND ANALYSIS DATA                       

Annual macroinvertebrate monitoring on the Upper Mississippi River System      
(UMRS) was implemented in 1992 under the Long Term Resource Monitoring Program 
(LTRMP).  Mayflies (Ephemeridae), fingernail clams (Sphaeriidae), and the      
exotic Corbicula species were selected for monitoring.  Midges (Chironomidae)  
were added to the monitoring scheme in 1993, with the addition of zebra        
mussels (Dreissena polymorpha) in 1995.   Mayflies, fingernail clams, and      
midges, found in the soft-sediment substrate, were chosen because they play an 
important ecological role in the UMRS.  The exotic Corbicula species and zebra 
mussel were chosen for sampling because of possible detrimental impacts they   
may have on the economy and biology of the UMRS.                               

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                                 FOREWARD                                      

Use of LTRMP Trend Data for Macroinvertebrate Monitoring                       

The sampling design for macroinvertebrates is relatively simple; however, all  
potential users of these data are strongly encouraged to read and understand   
the Macroinvertebrate Monitoring section of the LTRMP Procedures Manual (Thiel 
and Sauer 1999), which describes current procedures, before undertaking any    
analysis or interpretation of data.  This document is accessible by request    
from:                                                                          

          Macroinvertebrate Coordinator                                        
          Upper Midwest Environmental Sciences Center                          
          U.S. Geological Survey                                               
          2630 Fanta Reed Road                                                 
          La Crosse, WI  54603                                                 
          USA                                                                  
          Tel: (608) 783-7550, ext. 64                                         
          Fax: (608) 783-8058                                                  

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                                  SUMMARY                                      

LTRMP Macroinvertebrate Sampling Design                                        

The LTRMP conducts annual monitoring of macroinvertebrates in six study areas  
of the Upper Mississippi River System:  Navigation Pools 4, 8, 13 and          
26, and an unimpounded reach near Cape Girardeau, Missouri, on the Upper       
Mississippi River; and La Grange Pool on the Illinois River.  The LTRMP        
conducts highly standardized macroinvertebrate sampling in main channel        
border, side channel, contiguous (to the main channel or side channels)        
backwater, and impounded aquatic areas in each study areas.  Sampling is       
conducted using a standard Ponar grab dredge (Wildlife Supply Company,         
Saginaw, Michigan).                                                            

Sampling is based on a stratified random design and is conducted at            
approximately 125 sites in each study reach.  The aquatic areas serve a        
strata.  Sites include a few locations where benthic samples were collected    
historically.  Each field station is required to conduct macroinvertebrate     
sampling at the same time each year, prior to mayfly emergence.                

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                                REFERENCES                                     

Thiel, P. A., and J. S. Sauer.  1999.  Long Term Resource Monitoring Program   
Procedures:  Macroinvertebrate Monitoring.  U.S. Geological Survey,            
Upper Midwest Environmental Sciences Center, La Crosse, Wisconsin,             
Revised May 1999. LTRMP 95-P002-2 (Revised 1999) 7pp. + Appendixes A-H.        

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MACROINVERTEBRATE FIXED FORMAT FILE DOWNLOAD FILE

The fixed format file has all the data aligned to a specific column within the dataset.  
Every field will start and end exactly in the same location.  No field names are specified
in this format.       

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THE "FORMAT" COLUMN CONTAINS THE FOLLOWING FORMAT SPECIFIERS:                  
                       "a": alpha character (always UPPER case);               
                       "b": a blank space;                                     
                   "blank": a missing value;                                   
                       "c": alpha character/symbol/digit;                      
                   "c(xx)": character string where xx represents               
                            the maximum number of characters allowed;          
                       "9": any digit, 0 - 9;                                  
              "MM/DD/YYYY": date format;                                       

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Field Name                         Short Name          Format         Value Range/Default Value       Historical Info                                   

--------------------------------------------------------------------------------
BARCODE                            BARCODE             99999999       0 - 99999999                    1993 TO 2004                                      
SAMPLE_GRID_NUMBER                 GRID                99999                                          1992 TO 2004                                      
DATE_ONLY                          DATE                MM/DD/YYYY     05/01/1992 - Current Date       1992 TO 2004                                      
PROJECT_CODE                       PROJCODE            a-999                                          1992 TO 2004                                      
FIELD_STATION_NUMBER               FIELDSTA            9              0,1,2,3,4,5,6                   1992 TO 2004                                      
HABITAT_CLASS                      HABCLASS            aaa-a          (See Appendix)                  1992 TO 2004                                      
RIVER_MILE                         RM                  c99.9                                          1993 TO 2004                                      
SUBSAMPLE_TAKEN                    SUB                 c              N,A,B                           1994 TO 2004                                      
'15'                               ZONE15              99             15,16                           1992 TO 2004                                      
ZONE15_EAST_WEST                   EUTM15              999999                                         1992 TO 2004                                      
ZONE15_NORTH_SOUTH                 NUTM15              9999999                                        1992 TO 2004                                      
EMTC_UTM_ZONE                      EMTCZONE            99             15,16                           1992 TO 2004                                      
EMTC_UTM_EASTING                   EUTMEMTC            999999                                         1992 TO 2004                                      
EMTC_UTM_NORTHING                  NUTMEMTC            9999999                                        1992 TO 2004                                      
FIELD_UTM_ZONE                     FLDZONE             99             15,16                           1992 TO 2004                                      
FIELD_UTM_EASTING                  EFIELD              999999                                         1992 TO 2004                                      
FIELD_UTM_NORTHING                 NFIELD              9999999                                        1992 TO 2004                                      
FIELD_GPS_PDOP                     PDOP                99.9           0 - 99.9                        1992 TO 2004                                      
WATER_DEPTH                        DEPTH               99.9           0 - 99.9                        1992 TO 2004                                      
NUMBER_CONTAINERS                  NOCONT              99             1 - 99                          1993 TO 2004                                      
REPLICATE_A_CONTAINERS             REPA                9              1 - 9                           1992 TO 1992                                      
REPLICATE_B_CONTAINERS             REPB                9              1 - 9                           1992 TO 1992                                      
REPLICATE_C_CONTAINERS             REPC                9              1 - 9                           1992 TO 1992                                      
SUMMARY_CODE                       SUMCODE             9              1,2,3                           1992 TO 2004                                      
GEAR_CODE                          GEAR                a              (See Appendix)                  1992 TO 2004                                      
LAB_SAMPLE_TAKEN                   LAB                 a              Y,N                             1992 TO 2004                                      
SUBSTRATE_SAMPLE_TAKEN             SUBSAMPL            a              Y,N                             1992 TO 1992                                      
PCT_SUBMERSED_FLOAT_LEAF_VEG       SUBANDFL            9              0,1,2,3,4                       1992 TO 2004                                      
SUBSTRATE_CODE                     SUBSTRAT            9              0,1,2,3,4,5,6                   1992 TO 2004                                      
SHELLS                             SHELLS              c              Y,N                             1993 TO 2004                                      
DETRITUS                           DETRITUS            c              Y,N                             1993 TO 2004                                      
FINE_ORGANIC_MATERIAL              FINEORG             c              Y,N                             1993 TO 2004                                      
SITE_SUBMERSED_VEGETATION_CODE     SUBMERSE            9              0,1,2,3,4                       1992 TO 2004                                      
SITE_FLOATING_LEAF_CODE            FLOATING            9              0,1,2,3,4                       1992 TO 2004                                      
SITE_EMERGENT_VEGETATION_CODE      EMERGENT            9              0,1,2,3,4                       1992 TO 2004                                      
SITE_OPEN_WATER                    OPEN                9              0,1,2,3,4                       1992 TO 2004                                      
MAYFLIES_COUNT                     MAYFLIES            9999           0 - 9999                        1992 TO 2004                                      
FINGERNAIL_CLAMS_COUNT             FNC                 9999           0 - 9999                        1992 TO 2004                                      
CORBICULA_COUNT                    CORBICUL            9999           0 - 9999                        1992 TO 2004                                      
MIDGES_COUNT                       MIDGES              9999           0 - 9999                        1993 TO 2004                                      
ZEBRA_MUSSELS                      ZEBRA               9999           0 - 9999                        1995 TO 2004                                      
DRAGONFLIES_DOMSELFLIES            DRAGON              a              A,P                             1998 TO 2004                                      
STONEFLIES                         STONE               a              A,P                             1998 TO 2004                                      
CADDISFLIES                        CADDIS              a              A,P                             1998 TO 2004                                      
AQUATIC_FLIES                      FLIES               a              A,P                             1998 TO 2004                                      
FRESHWATER_MUSSELS                 MUSSELS             a              A,P                             1998 TO 2004                                      
AQUATIC_WORMS_LEECHES              WORMS               a              A,P                             1998 TO 2004                                      
FRESHWATER_SHRIMP_CRAYFISH         SHRIMP              a              A,P                             1998 TO 2004                                      
SCUDS                              SCUDS               a              A,P                             1998 TO 2004                                      
SNAILS                             SNAILS              a              A,P                             1998 TO 2004                                      
STATION_COMMENT                    COMMENTS            a(80)                                          1992 TO 2004                                      
RECORDER_CODE                      RCDCODE             9999           000 - 9999                      1992 TO 2004                                      
ENTRY_OPERATOR                     ENT_OPTR            ccc            000 - 999                       1992 TO 2001                                      
VERIFY_OPERATOR                    VRF_OPTR            ccc            000 - 999                       1992 TO 2001                                      
BATCH                              BATCH               9999                                           1992 TO 2004                                      
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Field Positions


Field Name                         Position
-----------------------------------------------------------------------------
BARCODE                            1:8
SAMPLE_GRID_NUMBER                 9:13
DATE_ONLY                          14:23
PROJECT_CODE                       24:28
FIELD_STATION_NUMBER               29:29
HABITAT_CLASS                      30:34
RIVER_MILE                         35:39
SUBSAMPLE_TAKEN                    40:40
UTM_ZONE_LITERAL                   41:42
ZONE15_EAST_WEST                   43:48
ZONE15_NORTH_SOUTH                 49:55
EMTC_UTM_ZONE                      56:57
EMTC_UTM_EASTING                   58:63
EMTC_UTM_NORTHING                  64:70
FIELD_UTM_ZONE                     71:72
FIELD_UTM_EASTING                  73:78
FIELD_UTM_NORTHING                 79:85
FIELD_GPS_PDOP                     86:89
WATER_DEPTH                        90:93
NUMBER_CONTAINERS                  94:95
REPLICATE_A_CONTAINERS             96:96
REPLICATE_B_CONTAINERS             97:97
REPLICATE_C_CONTAINERS             98:98
SUMMARY_CODE                       99:99
GEAR_CODE                          100:100
LAB_SAMPLE_TAKEN                   101:101
SUBSTRATE_SAMPLE_TAKEN             102:102
PCT_SUBMERSED_FLOAT_LEAF_VEG       103:103
SUBSTRATE_CODE                     104:104
SHELLS                             105:105
DETRITUS                           106:106
FINE_ORGANIC_MATERIAL              107:107
SITE_SUBMERSED_VEGETATION_CODE     108:108
SITE_FLOATING_LEAF_CODE            109:109
SITE_EMERGENT_VEGETATION_CODE      110:110
SITE_OPEN_WATER                    111:111
MAYFLIES_COUNT                     112:115
FINGERNAIL_CLAMS_COUNT             116:119
CORBICULA_COUNT                    120:123
MIDGES_COUNT                       124:127
ZEBRA_MUSSELS                      128:131
DRAGONFLIES_DOMSELFLIES            132:132
STONEFLIES                         133:133
CADDISFLIES                        134:134
AQUATIC_FLIES                      135:135
FRESHWATER_MUSSELS                 136:136
AQUATIC_WORMS_LEECHES              137:137
FRESHWATER_SHRIMP_CRAYFISH         138:138
SCUDS                              139:139
SNAILS                             140:140
STATION_COMMENT                    141:220
RECORDER_CODE                      221:224
ENTRY_OPERATOR                     225:227
VERIFY_OPERATOR                    228:230
BATCH                              231:234
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Field Descriptions



BARCODE
Identifying barcode for invertebrate data sheets.



SAMPLE GRID
Sample grid area for a collection site. Grid numbers are unique descriptors
for each sample site location, and are derived from a pre-determined UTM
grid system developed at UMESC.



DATE
The month, day, and year the site was sampled. Format is mm/dd/yyyy.



PROJECT CODE
Alphanumeric LTRMP project code. Format is A-nnn, where A is a letter
describing project type and nnn is a special project number. Project types are
as follows:
M = RTA standardized resource monitoring
B = HREP biological response monitoring
E = Ad hoc exploratory sampling
R = Special research project



FIELD STATION NUMBER
Numeric code identifying the field station number. Listed below are the
valid codes and their corresponding field station.
1 = Lake City, MN 4 = Brighton, IL
2 = Onalaska, WI 5 = Jackson, MO
3 = Bellevue, IA 6 = Havana, IL



HABITAT CLASS
Alphabetic code LTRMP habitat class description.
Valid codes are as follows:
BWC = Backwater contiguous
IMP = Impounded
MC = Main channel
MCB = Main channel border
SC = Side channel
TDL = Tributary delta lake (Lake Pepin only)



RIVER MILE
The location of the site to the nearest tenth of a river mile.



SUBSAMPLE TAKEN
A Code describing if a subsample was taken
Valid codes are as follows:
1994 TO 1996 only A = Large, Subsample(268 square centimeteters)
B = Small, Subsample(162 square centimeteters)
N = No, Subsample Taken
1997 - TO PRESENT Y = Yes, Subsample Taken
N = No, Subsample Taken



UTM ZONE (ZONE 15)
Numeric field to record Global Positioning System (GPS) zone.
LTRMP data resides in either zone 15 or 16. If the GPS zone = 16
the GPS zone is converted to zone 15.



UTM EASTING (ZONE 15)
East UTM (Universal Transverse Mercator) coordinates of
collection sites. This coordinate is computed at UMESC and
is then used by the collection. This is the coordinate recorded
in the field and converted to zone 15.



UTM NORTHING (ZONE 15)
North UTM (Universal Transverse Mercator) coordinates of collection sites.
This coordinate is computed at UMESC and is then used by the collection.
This is the coordinate recorded in the field and converted to zone 15.



UTM ZONE (UMESC)
Numeric field to record Global Positioning System (GPS) zone. LTRMP data
resides in either zone 15 or 16.
This zone is derived at UMESC and is then used by the collection crews
to locate where they are to do their sampling.



UTM EASTING (UMESC)
East UTM (Universal Transverse Mercator) coordinates of collection sites.
This coordinate is derived at UMESC and is then used by the collection
crews to locate where they are to do their sampling.



UTM NORTHING (UMESC)
North UTM (Universal Transverse Mercator) coordinates of collection sites.
This coordinate is derived at UMESC and is then used by the collection
crews to locate where they are to do their sampling.



UTM ZONE (FIELD)
Numeric field to record Global Positioning System (GPS) zone.
LTRMP data resides in either zone 15 or 16. This zone is derived
in the field when the collection crew can not sample the
(UMESC derived) collection site. This is the actual location
of the collection site used by the collection crews.



UTM EASTING (FIELD)
East UTM (Universal Transverse Mercator) coordinates of collection sites.
This coordinate is derived in the field when the collection crew can
not sample the (UMESC derived) collection site. This is the actual
location of the collection site used by the collection crews.



UTM NORTHING (FIELD)
North UTM (Universal Transverse Mercator) coordinates of collection sites.
This coordinate is derived in the field when the collection crew can not
sample the (UMESC derived) collection site. This is the actual location
of the collection site used by the collection crews.



ACCURACY
Numeric field to record a measure of positioning accuracy. Record Percent
Dilution of Precision (PDOP) from GPS devices and the following codes for base
map cross-reference and revisits to marked fixed sites:
00.1 = Almost certain accuracy within 100 m because of immediate proximity to
uniquely identifiable features (undisturbed marker, wing dam, day mark,
etc.).
00.2 = High confidence of accuracy within 100 m because of agreement between
general site appearance and identifiable features on a base map.
00.3 = Other than above.



DEPTH
Water depth recorded in meters.



CONTAINERS
The total number of sample containers collected at the site and taken back to
the lab (replaced # containers per replicate A, B, C in 1993).



CONTAINERS PER REPLICATE - A
The number of sample containers collected at the site and taken back to
the lab for the first sample taken at a site (applies to 1992 only).



CONTAINERS PER REPLICATE - B
The number of sample containers collected at the site and taken back to
the lab for the second sample taken at a site (applies to 1992 only).



CONTAINERS PER REPLICATE - C
The number of sample containers collected at the site and taken back to
the lab for the third sample taken at a site (applies to 1992 only).



SUMMARY CODE
A numeric code representing whether or not the sample was kept and the
circumstances surrounding the decision to accept or reject. Valid codes
are as follows:
1 = Sample taken
2 = Sample taken but not kept due to hard substrate
3 = Sample taken but not kept due to detritus, shells, etc.
4 = Other



GEAR CODE
A code representing the gear type used to collect the sample.
(P = Standard Ponar).
(E = Petite Ponar)



LAB SAMPLE TAKEN
Code if the sample was chosen for lab analysis. Valid codes are as
follows:
Y = Yes, sample chosen for lab analysis.
N = No, sample not chosen for lab analysis.



SUBSTRATE SAMPLE TAKEN
A code describing if a substrate sample was taken.
Valid codes are as follows (Applies to 1992 only):
Y = Yes substrate sample taken
N = No substrate sample taken.



% SUBMERSED AND FLOATING LEAF VEGETATION (Ponar)
This visual observation is a subjective measurement based on
the column of water and sediment the Ponar falls through. A
numeric code is recorded representing the percentage
of submersed and floating-leaf vegetation.
This field will provide data on whether or not invertebrates
associated with vegetation are in the sample and the relationship of
sites with and without vegetation.
0 = 0%
1 = 1%-20%
2 = 21%-50%
3 = 51%-90%
4 = 91%-100%



PREDOMINANT SUBSTRATE
A code to represent the qualitative observation of
sediments based on visual and tactile observations at the site.
Valid codes are as follows:
1 = Hard clay
2 = Silt/Clay
3 = Mostly silt/clay with sand
4 = Mostly sand
5 = Sand
6 = Gravel/rock



SHELLS
A code representing the percentage of dead shells in the sample
wash frame. Valid codes are as follows:
0 = 0%
1 = 1%-20%
2 = 21%-50%
3 = 51%-90%
4 = 91%-100%



DETRITUS
A code representing the percentage of detritus (fibrous
material on the surface and within the sediment) in the sample
wash frame. Valid codes are as follows:
0 = 0%
1 = 1%-20%
2 = 21%-50%
3 = 51%-90%
4 = 91%-100%



FINE ORGANIC MATERIAL
A code indicating if fine organic material (characterized by black
coloration and odor) is present in the sample.
Valid codes are as follows:
Y = Yes, fine organic material present
N = No, fine organic material not present



SUBMERSED VEGETATION
A code representing the percentage of submersed vegetation
within a 15-m radius from the boat. Valid codes are as follows:
0 = 0%
1 = 1%-20%
2 = 21%-50%
3 = 51%-90%
4 = 91%-100%



FLOATING LEAF
A code representing the percentage of floating leaf vegetation
within a 15-m radius from the boat. Valid codes are as follows:
0 = 0%
1 = 1%-20%
2 = 21%-50%
3 = 51%-90%
4 = 91%-100%



EMERGENT VEGETATION
A code representing the percentage of emergent vegetation
within a 15-m radius from the boat. Valid codes are as follows:
0 = 0%
1 = 1%-20%
2 = 21%-50%
3 = 51%-90%
4 = 91%-100%



OPEN WATER
A code representing the percentage of open water
within a 15-m radius from the boat. Valid codes are as follows:
0 = 0%
1 = 1%-20%
2 = 21%-50%
3 = 51%-90%
4 = 91%-100%



MAYFLIES
The number of mayfly larvae found in the collection sample for
the site.



FINGERNAIL CLAMS
The number of fingernail clams found in the collection sample for
the site.



CORBICULA
The number of corbicula found in the collection sample for
the site.



MIDGES
The number of midges found in the collection sample for
the site.



ZEBRA MUSSELS
The number of zebra mussels found in the collection sample for
the site (not started recording until 1995).



DRAGONFLIES DAMSELFLIES
A code representing if dragonflies or damselflies are absent (A) or
present (P) in the sample (not started recording until 1998).



STONEFLIES
A code representing if stoneflies are absent (A) or
present (P) in the sample (not started recording until 1998).



CADDISFLIES
A code representing if caddisflies are absent (A) or
present (P) in the sample (not started recording until 1998).



AQUATIC FLIES
A code representing if aquatic flies are absent (A) or
present (P) in the sample (not started recording until 1998).



FRESHWATER MUSSELS
A code representing if fresh water mussels are absent (A) or
present (P) in the sample (not started recording until 1998).



AQUATIC WORMS LEECHES
A code representing if aquatic worms or leeches are absent (A) or
present (P) in the sample (not started recording until 1998).



FRESHWATER SHRIMP CRAYFISH
A code representing if fresh water shrimp or crayfish are absent (A) or
present (P) in the sample (not started recording until 1998).



SCUDS
A code representing if scuds are absent (A) or
present (P) in the sample (not started recording until 1998).



SNAILS
A code representing if snails are absent (A) or
present (P) in the sample (not started recording until 1998).



COMMENTS
Eighty-character field to record miscellaneous comments and
observations.



RECORDER CODE
This number uniquely identifies the person who coded
the data sheet.



ENTRY OPERATOR
Alphanumeric field to identify the operator responsible for entering (keying)
the invertebrate data into an electronic format.



VERIFY OPERATOR
Alphanumeric field to identify the operator responsible for verifying the
electronic keyed invertebrate data.



BATCH NUMBER
Administrative field used to track keyed data batches.


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