MACROINVERTEBRATE LIABILITY DISCLAIMER AND FORMAT DESCRIPTION Disclaimer These data have been generated by State agencies under contract with the Department of the Interior and the Upper Midwest Environmental Sciences Center (UMESC). Although these data have been processed successfully on a computer system at the UMESC, no warranty, expressed or implied, is made regarding the accuracy or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. Error correction is a continuing process, and UMESC corrects data data errors routinely when a correct value can be determined. UMESC does not delete questionable data when the correct value cannot be determined, but attempts to mark such data with an appropriate flag. Users are strongly cautioned to check the accuracy of any data obtained and to report suspected values to UMESC. Methods, purposes, and conditions can vary dramatically among data-collecting efforts and these data should not be combined with other datasets from the UMESC or other sources unless the user fully understands the procedures and purposes used to create each set of data and can verify that data are comparable across sources. ------------------------------------------------------------------------------- MACROINVERTEBRATE TREND ANALYSIS DATA Annual macroinvertebrate monitoring on the Upper Mississippi River System (UMRS) was implemented in 1992 under the Long Term Resource Monitoring Program (LTRMP). Mayflies (Ephemeridae), fingernail clams (Sphaeriidae), and the exotic Corbicula species were selected for monitoring. Midges (Chironomidae) were added to the monitoring scheme in 1993, with the addition of zebra mussels (Dreissena polymorpha) in 1995. Mayflies, fingernail clams, and midges, found in the soft-sediment substrate, were chosen because they play an important ecological role in the UMRS. The exotic Corbicula species and zebra mussel were chosen for sampling because of possible detrimental impacts they may have on the economy and biology of the UMRS. ------------------------------------------------------------------------------ FOREWARD Use of LTRMP Trend Data for Macroinvertebrate Monitoring The sampling design for macroinvertebrates is relatively simple; however, all potential users of these data are strongly encouraged to read and understand the Macroinvertebrate Monitoring section of the LTRMP Procedures Manual (Thiel and Sauer 1999), which describes current procedures, before undertaking any analysis or interpretation of data. This document is accessible by request from: Macroinvertebrate Coordinator Upper Midwest Environmental Sciences Center U.S. Geological Survey 2630 Fanta Reed Road La Crosse, WI 54603 USA Tel: (608) 783-7550, ext. 64 Fax: (608) 783-8058 ------------------------------------------------------------------------------ SUMMARY LTRMP Macroinvertebrate Sampling Design The LTRMP conducts annual monitoring of macroinvertebrates in six study areas of the Upper Mississippi River System: Navigation Pools 4, 8, 13 and 26, and an unimpounded reach near Cape Girardeau, Missouri, on the Upper Mississippi River; and La Grange Pool on the Illinois River. The LTRMP conducts highly standardized macroinvertebrate sampling in main channel border, side channel, contiguous (to the main channel or side channels) backwater, and impounded aquatic areas in each study areas. Sampling is conducted using a standard Ponar grab dredge (Wildlife Supply Company, Saginaw, Michigan). Sampling is based on a stratified random design and is conducted at approximately 125 sites in each study reach. The aquatic areas serve a strata. Sites include a few locations where benthic samples were collected historically. Each field station is required to conduct macroinvertebrate sampling at the same time each year, prior to mayfly emergence. ------------------------------------------------------------------------------ REFERENCES Thiel, P. A., and J. S. Sauer. 1999. Long Term Resource Monitoring Program Procedures: Macroinvertebrate Monitoring. U.S. Geological Survey, Upper Midwest Environmental Sciences Center, La Crosse, Wisconsin, Revised May 1999. LTRMP 95-P002-2 (Revised 1999) 7pp. + Appendixes A-H. ------------------------------------------------------------------------------- INVERTEBRATE FIXED FILE FORMAT ALL FIELDS ARE RIGHT JUSTIFIED IN ASCII CHARACTER FORMAT. 1992 DATA OR EARLIER IS SORTED BY DATE, SAMPLE GRID, UTM EASTING, AND UTM NORTHING IN THAT ORDER. 1993 DATA OR LATER IS SORTED BY BARCODE ONLY. THE "FORMAT" COLUMN CONTAINS THE FOLLOWING FORMAT SPECIFIERS: "a": alpha character (always UPPER case); "b": a blank space; "blank": a missing value; "c": alpha character/symbol/digit; "c(xx)": character string where xx represents the maximum numberof characters allowed; "9": any digit, 0 - 9; "MM/DD/YYYY": date format; "HH:MI": time format; FIELD FIELD VALUE RANGE / NAME POSITION FORMAT DEFAULT VALUE ---------- -------- ------ ------------- BARCODE 1:8 99999999 0 - 99999999 SAMPLE GRID 9:13 99999 SEE FIELD DESCRIPTIONS DATE 14:23 MM/DD/YYYY 05/01/1992 - TO PRESENT PROJECT CODE 24:28 a-999 SEE FIELD DESCRIPTIONS FIELD STATION # 29:29 9 0,1,2,3,4,5,6 HABITAT CLASS 30:34 aaa-a SEE FIELD DESCRIPTIONS RIVER MILE 35:39 c99.9 SEE FIELD DESCRIPTIONS SUBSAMPLE TAKEN 40:40 c N,A,B UTM ZONE (ZONE 15) 41:42 99 15,16 UTM EASTING (ZONE 15) 43:48 999999 SEE FIELD DESCRIPTIONS UTM NORTHING (ZONE 15) 49:55 9999999 SEE FIELD DESCRIPTIONS UTM ZONE (EMTC) 56:57 99 15,16 UTM EASTING (EMTC) 58:63 999999 SEE FIELD DESCRIPTIONS UTM NORTHING (EMTC) 64:70 9999999 SEE FIELD DESCRIPTIONS UTM ZONE (FIELD) 71:72 99 15,16 UTM EASTING (FIELD) 73:78 999999 SEE FIELD DESCRIPTIONS UTM NORTHING (FIELD) 79:85 9999999 SEE FIELD DESCRIPTIONS ACCURACY 86:89 99.9 0 - 99.9 DEPTH 90:93 99.9 0 - 99.9 # CONTAINERS 94:95 99 1 - 99 # CONTAINERS PER REPLICATE - A 96:96 9 1 - 9 # CONTAINERS PER REPLICATE - B 97:97 9 1 - 9 # CONTAINERS PER REPLICATE - C 98:98 9 1 - 9 SUMMARY CODE 99:99 9 1,2,3 GEAR CODE 100:100 a SEE FIELD DESCRIPTIONS LAB SAMPLE TAKEN 101:101 a Y,N SUBSTRATE SAMPLE TAKEN 102:102 a Y,N % SUBMERSED AND FLOATING LEAF VEGETATION (Ponar) 103:103 9 0,1,2,3,4 PREDOMINANT SUBSTRATE 104:104 9 0,1,2,3,4,5,6 SHELLS 105:105 c Y,N DETRITUS 106:106 c Y,N FINE ORGANIC MATERIAL 107:107 c Y,N SUBMERSED VEGETATION 108:108 9 0,1,2,3,4 FLOATING LEAF 109:109 9 0,1,2,3,4 EMERGENT VEGETATION 110:110 9 0,1,2,3,4 OPEN WATER 111:111 9 0,1,2,3,4 MAYFLIES 112:115 9999 0 - 9999 FINGERNAIL CLAMS 116:119 9999 0 - 9999 CORBICULA 120:123 9999 0 - 9999 MIDGES 124:127 9999 0 - 9999 ZEBRA MUSSELS 128:131 9999 0 - 9999 DRAGONFLIES DAMSELFLIES 132:132 a A,P STONEFLIES 133:133 a A,P CADDISFLIES 134:134 a A,P AQUATIC FLIES 135:135 a A,P FRESHWATER MUSSELS 136:136 a A,P AQUATIC WORMS LEECHES 137:137 a A,P FRESHWTR SHRIMP CRAYFISH 138:138 a A,P SCUDS 139:139 a A,P SNAILS 140:140 a A,P COMMENTS 141:220 a(80) SEE FIELD DESCRIPTIONS RECORDER CODE 221:224 9999 000 - 9999 ENTRY OPERATOR 225:227 ccc 000 - 999 VERIFY OPERATOR 228:230 ccc 000 - 999 BATCH NUMBER 231:234 9999 ------------------------------------------------------------------------------ HISTORICAL SUMMARY FIELD YEARS NAME USED ---------- ----- BARCODE 93 - TO PRESENT SAMPLE GRID 92 - TO PRESENT DATE 92 - TO PRESENT PROJECT CODE 92 - TO PRESENT FIELD STATION # 92 - TO PRESENT HABITAT CLASS 92 - TO PRESENT RIVER MILE 93 - TO PRESENT SUBSAMPLE TAKEN 94 - TO PRESENT UTM ZONE (ZONE 15) 92 - TO PRESENT UTM EASTING (ZONE 15) 92 - TO PRESENT UTM NORTHING (ZONE 15) 92 - TO PRESENT UTM ZONE (EMTC) 92 - TO PRESENT UTM EASTING (EMTC) 92 - TO PRESENT UTM NORTHING (EMTC) 92 - TO PRESENT UTM ZONE (FIELD) 92 - TO PRESENT UTM EASTING (FIELD) 92 - TO PRESENT UTM NORTHING (FIELD) 92 - TO PRESENT ACCURACY 92 - TO PRESENT DEPTH 92 - TO PRESENT # CONTAINERS 93 - TO PRESENT # CONTAINERS PER REPLICATE - A 92 # CONTAINERS PER REPLICATE - B 92 # CONTAINERS PER REPLICATE - C 92 SUMMARY CODE 92 - TO PRESENT GEAR CODE 92 - TO PRESENT LAB SAMPLE TAKEN 92 - TO PRESENT SUBSTRATE SAMPLE TAKEN 92 % SUBMERSED AND FLOATING 92 - TO PRESENT LEAF VEGETATION (Ponar) 92 - TO PRESENT PREDOMINANT SUBSTRATE 92 - TO PRESENT SHELLS 93 - TO PRESENT DETRITUS 93 - TO PRESENT FINE ORGANIC MATERIAL 93 - TO PRESENT SUBMERSED VEGETATION 92 - TO PRESENT FLOATING LEAF 92 - TO PRESENT EMERGENT VEGETATION 92 - TO PRESENT OPEN WATER 92 - TO PRESENT MAYFLIES 92 - TO PRESENT FINGERNAIL CLAMS 92 - TO PRESENT CORBICULA 92 - TO PRESENT MIDGES 93 - TO PRESENT ZEBRA MUSSELS 95 - TO PRESENT DRAGONFLIES DAMSELFLIES 98 - TO PRESENT STONEFLIES 98 - TO PRESENT CADDISFLIES 98 - TO PRESENT AQUATIC FLIES 98 - TO PRESENT FRESHWATER MUSSELS 98 - TO PRESENT AQUATIC WORMS LEECHES 98 - TO PRESENT FRESHWATER SHRIMP CRAYFISH 98 - TO PRESENT SCUDS 98 - TO PRESENT SNAILS 98 - TO PRESENT COMMENTS 92 - TO PRESENT RECORDER CODE 92 - TO PRESENT ENTRY OPERATOR 92 - TO PRESENT VERIFY OPERATOR 92 - TO PRESENT BATCH NUMBER 92 - TO PRESENT -------------------------------------------------------------------------------- FIELD DESCRIPTIONS BARCODE Identifying barcode for invertebrate data sheets. SAMPLE GRID Sample grid area for a collection site. Grid numbers are unique descriptors for each sample site location, and are derived from a pre-determined UTM grid system developed at EMTC. DATE The month, day, and year the site was sampled. Format is mm/dd/yyyy. PROJECT CODE Alphanumeric LTRMP project code. Format is A-nnn, where A is a letter describing project type and nnn is a special project number. Project types are as follows: M = RTA standardized resource monitoring B = HREP biological response monitoring E = Ad hoc exploratory sampling R = Special research project FIELD STATION # Numeric code identifying the field station number. Listed below are the valid codes and their corresponding field station. 1 = Lake City, MN 4 = Brighton, IL 2 = Onalaska, WI 5 = Jackson, MO 3 = Bellevue, IA 6 = Havana, IL HABITAT CLASS Alphabetic code LTRMP habitat class description. Valid codes are as follows: BWC = Backwater contiguous IMP = Impounded MC = Main channel MCB = Main channel border SC = Side channel TDL = Tributary delta lake (Lake Pepin only) RIVER MILE The location of the site to the nearest tenth of a river mile. SUBSAMPLE TAKEN A Code describing if a subsample was taken Valid codes are as follows: 1994 TO 1996 only A = Large, Subsample(268 square centimeteters) B = Small, Subsample(162 square centimeteters) N = No, Subsample Taken 1997 - TO PRESENT Y = Yes, Subsample Taken N = No, Subsample Taken UTM ZONE (ZONE 15) Numeric field to record Global Positioning System (GPS) zone. LTRMP data resides in either zone 15 or 16. If the GPS zone = 16 the GPS zone is converted to zone 15. UTM EASTING (ZONE 15) East UTM (Universal Transverse Mercator) coordinates of collection sites. This coordinate is computed at EMTC and is then used by the collection. This is the coordinate recorded in the field and converted to zone 15. UTM NORTHING (ZONE 15) North UTM (Universal Transverse Mercator) coordinates of collection sites. This coordinate is computed at EMTC and is then used by the collection. This is the coordinate recorded in the field and converted to zone 15. UTM ZONE (EMTC) Numeric field to record Global Positioning System (GPS) zone. LTRMP data resides in either zone 15 or 16. This zone is derived at EMTC and is then used by the collection crews to locate where they are to do their sampling. UTM EASTING (EMTC) East UTM (Universal Transverse Mercator) coordinates of collection sites. This coordinate is derived at EMTC and is then used by the collection crews to locate where they are to do their sampling. UTM NORTHING (EMTC) North UTM (Universal Transverse Mercator) coordinates of collection sites. This coordinate is derived at EMTC and is then used by the collection crews to locate where they are to do their sampling. UTM ZONE (FIELD) Numeric field to record Global Positioning System (GPS) zone. LTRMP data resides in either zone 15 or 16. This zone is derived in the field when the collection crew can not sample the (EMTC derived) collection site. This is the actual location of the collection site used by the collection crews. UTM EASTING (FIELD) East UTM (Universal Transverse Mercator) coordinates of collection sites. This coordinate is derived in the field when the collection crew can not sample the (EMTC derived) collection site. This is the actual location of the collection site used by the collection crews. UTM NORTHING (FIELD) North UTM (Universal Transverse Mercator) coordinates of collection sites. This coordinate is derived in the field when the collection crew can not sample the (EMTC derived) collection site. This is the actual location of the collection site used by the collection crews. ACCURACY Numeric field to record a measure of positioning accuracy. Record Percent Dilution of Precision (PDOP) from GPS devices and the following codes for base map cross-reference and revisits to marked fixed sites: 00.1 = Almost certain accuracy within 100 m because of immediate proximity to uniquely identifiable features (undisturbed marker, wing dam, day mark, etc.). 00.2 = High confidence of accuracy within 100 m because of agreement between general site appearance and identifiable features on a base map. 00.3 = Other than above. DEPTH Water depth recorded in meters. # CONTAINERS The total number of sample containers collected at the site and taken back to the lab (replaced # containers per replicate A, B, C in 1993). # CONTAINERS PER REPLICATE - A The number of sample containers collected at the site and taken back to the lab for the first sample taken at a site (applies to 1992 only). # CONTAINERS PER REPLICATE - B The number of sample containers collected at the site and taken back to the lab for the second sample taken at a site (applies to 1992 only). # CONTAINERS PER REPLICATE - C The number of sample containers collected at the site and taken back to the lab for the third sample taken at a site (applies to 1992 only). SUMMARY CODE A numeric code representing whether or not the sample was kept and the circumstances surrounding the decision to accept or reject. Valid codes are as follows: 1 = Sample taken 2 = Sample taken but not kept due to hard substrate 3 = Sample taken but not kept due to detritus, shells, etc. 4 = Other GEAR CODE A code representing the gear type used to collect the sample. (P = Standard Ponar). (E = Petite Ponar) LAB SAMPLE TAKEN Code if the sample was chosen for lab analysis. Valid codes are as follows: Y = Yes, sample chosen for lab analysis. N = No, sample not chosen for lab analysis. SUBSTRATE SAMPLE TAKEN A code describing if a substrate sample was taken. Valid codes are as follows (Applies to 1992 only): Y = Yes substrate sample taken N = No substrate sample taken. % SUBMERSED AND FLOATING LEAF VEGETATION (Ponar) This visual observation is a subjective measurement based on the column of water and sediment the Ponar falls through. A numeric code is recorded representing the percentage of submersed and floating-leaf vegetation. This field will provide data on whether or not invertebrates associated with vegetation are in the sample and the relationship of sites with and without vegetation. 0 = 0% 1 = 1%-20% 2 = 21%-50% 3 = 51%-90% 4 = 91%-100% PREDOMINANT SUBSTRATE A code to represent the qualitative observation of sediments based on visual and tactile observations at the site. Valid codes are as follows: 1 = Hard clay 2 = Silt/Clay 3 = Mostly silt/clay with sand 4 = Mostly sand 5 = Sand 6 = Gravel/rock SHELLS A code representing the percentage of dead shells in the sample wash frame. Valid codes are as follows: 0 = 0% 1 = 1%-20% 2 = 21%-50% 3 = 51%-90% 4 = 91%-100% DETRITUS A code representing the percentage of detritus (fibrous material on the surface and within the sediment) in the sample wash frame. Valid codes are as follows: 0 = 0% 1 = 1%-20% 2 = 21%-50% 3 = 51%-90% 4 = 91%-100% FINE ORGANIC MATERIAL A code indicating if fine organic material (characterized by black coloration and odor) is present in the sample. Valid codes are as follows: Y = Yes, fine organic material present N = No, fine organic material not present SUBMERSED VEGETATION A code representing the percentage of submersed vegetation within a 15-m radius from the boat. Valid codes are as follows: 0 = 0% 1 = 1%-20% 2 = 21%-50% 3 = 51%-90% 4 = 91%-100% FLOATING LEAF A code representing the percentage of floating leaf vegetation within a 15-m radius from the boat. Valid codes are as follows: 0 = 0% 1 = 1%-20% 2 = 21%-50% 3 = 51%-90% 4 = 91%-100% EMERGENT VEGETATION A code representing the percentage of emergent vegetation within a 15-m radius from the boat. Valid codes are as follows: 0 = 0% 1 = 1%-20% 2 = 21%-50% 3 = 51%-90% 4 = 91%-100% OPEN WATER A code representing the percentage of open water within a 15-m radius from the boat. Valid codes are as follows: 0 = 0% 1 = 1%-20% 2 = 21%-50% 3 = 51%-90% 4 = 91%-100% MAYFLIES The number of mayfly larvae found in the collection sample for the site. FINGERNAIL CLAMS The number of fingernail clams found in the collection sample for the site. CORBICULA The number of corbicula found in the collection sample for the site. MIDGES The number of midges found in the collection sample for the site. ZEBRA MUSSELS The number of zebra mussels found in the collection sample for the site (not started recording until 1995). DRAGONFLIES DAMSELFLIES A code representing if dragonflies or damselflies are absent (A) or present (P) in the sample (not started recording until 1998). STONEFLIES A code representing if stoneflies are absent (A) or present (P) in the sample (not started recording until 1998). CADDISFLIES A code representing if caddisflies are absent (A) or present (P) in the sample (not started recording until 1998). AQUATIC FLIES A code representing if aquatic flies are absent (A) or present (P) in the sample (not started recording until 1998). FRESHWATER MUSSELS A code representing if fresh water mussels are absent (A) or present (P) in the sample (not started recording until 1998). AQUATIC WORMS LEECHES A code representing if aquatic worms or leeches are absent (A) or present (P) in the sample (not started recording until 1998). FRESHWATER SHRIMP CRAYFISH A code representing if fresh water shrimp or crayfish are absent (A) or present (P) in the sample (not started recording until 1998). SCUDS A code representing if scuds are absent (A) or present (P) in the sample (not started recording until 1998). SNAILS A code representing if snails are absent (A) or present (P) in the sample (not started recording until 1998). COMMENTS Eighty-character field to record miscellaneous comments and observations. RECORDER CODE This number uniquely identifies the person who coded the data sheet. ENTRY OPERATOR Alphanumeric field to identify the operator responsible for entering (keying) the invertebrate data into an electronic format. VERIFY OPERATOR Alphanumeric field to identify the operator responsible for verifying the electronic keyed invertebrate data. BATCH NUMBER Administrative field used to track keyed data batches. --------------------------------------------------------------------------------