Meinertz, J.R., S.L. Greseth, and T.M. Schreier.  2004. 
Determination of the optimal water temperature, isoeugenol concentration, 
and exposure duration that produce the maximum isoeugenol concentration 
in rainbow trout skin-on fillet tissue.  Submitted to the UMESC archives
August 25, 2004.  116 pages.

Summary
The objective of the study was to determine the exposure conditions (water 
temperature, isoeugenol concentration, and exposure duration) that would generate the 
greatest isoeugenol concentration in the edible fillet of rainbow trout.  Rainbow trout 
were exposed to isoeugenol in water temperatures of 7, 12, and 17 °C, isoeugenol 
concentrations near 7 and 17 mg/L, and exposure durations of 10 and 60 min.  All 
exposures were static.  Immediately after the end of an exposure, fish were rinsed, 
sacrificed, and skin-on fillets removed.  The fillets were homogenized with dry ice and 
the homogenate stored at less than -70 °C until the tissue was extracted with 
acetonitrile and the extracts analyzed for isoeugenol by liquid chromatography with 
absorbance detection.  Although the maximum isoeuegnol concentration found in fillet 
tissue (78.8 ug/g) was generated with a water temperature of 17 °C, an AQUI-S™
water concentration near 14 mg/L, and an exposure duration of 60 min, the concentration was 
not significantly greater than the tissue concentration (70.7 ug/g) generated at 12 °C 
with the same exposure conditions (P < 0.01).