Vue, C., Schmidt, L. J., Stehly, G. R., and Gingerich, W. H., 2002, Liquid
chromatographic determination of florfenicol in the plasma of multiple species of fish:
Journal of Chromatography B Analytical Technologies in the Biomedical and Life
Sciences, v. 780, no. 1, p. 111-117. 

Abstract

A simple method was developed for determining florfenicol concentration in a small
volume (250 mul) of plasma from five phylogenetically diverse species of freshwater
fish. Florfenicol was isolated from the plasma matrix through C-18 solid-phase
extraction and quantified by reversed-phase high-performance liquid chromatography
with UV detection. The accuracy (84-104%), precision (%RSDless than or equal to8),
and sensitivity (quantitation limit <30 ng/ml) of the method indicate its usefulness for
conducting pharmacokinetic studies on a variety of freshwater fish.

Keywords

fish/florfenicol, Salmo-salar L, Atlantic salmon, chloramphenicol, thiamphenicol,
efficacy