Meinertz, J. R., Stehly, G. R., Hubert, T. D., and Bernardy, J. A., 1999, Liquid
chromatographic determination of benzocaine and N-acetylbenzocaine in the edible
fillet tissue from rainbow trout: Journal of Chromatography A, v. 855, no. 1, p.
255-260.

Abstract

A method was developed for determining benzocaine and N-acetylbenzocaine
concentrations in fillet tissue of rainbow trout. The method involves extracting the
analytes with acetonitrile, removing lipids or hydrophobic compounds from the
extract with hexane, and providing additional clean-up with solid-phase extraction
techniques. Analyte concentrations are determined using reversed-phase
high-performance liquid chromatographic techniques with an isocratic mobile phase
and UV detection. The accuracy (range, 92 to 121%), precision (R.S.D., <14%), and
sensitivity (method quantitation limit, <24 ng/g) for each analyte indicate the
usefulness of this method for studies characterizing the depletion of benzocaine
residues from fish exposed to benzocaine. (C) 1999 Published by Elsevier Science
B.V. All rights reserved.

Keywords

fish, food analysis, benzocaine, acetylbenzocaine, BASS