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Protocols

List of study protocols for various technical sections

Contact the study director listed next to the protocol title for assistance.

Efficacy

Elimination of Renibacterium salmoninarum, the causative agent of bacterial kidney disease (BKD), from salmonids following administration of enrofloxacin or florfenicol.  Mark P. Gaikowski. 

Field effectiveness of Aquaflor® (50% Type A Medicated Article), Florfenicol - SCH25298 administration in feed to control mortality associated with Streptococcus iniae in tilapia (Oreochromis spp).  Mark P. Gaikowski. 

Identification of methods to consistently produce external columnaris infections on freshwater fish.  Mark P. Gaikowski.

Study protocol for a compassionate aquaculture Investigational New Animal Drug Exemption (INAD #10-023).  Clinical field trials to determine the efficacy of Perox-Aid™ to control or prevent mortalities associated with bacterial gill disease and external flavobacterial infections in a variety of cultured fish.  Mark P. Gaikowski and Jeff J. Rach. 

Study protocol for a compassionate aquaculture Investigational New Animal Drug Exemption (INAD #10-023).  Clinical field trials to determine the efficacy of Perox-Aid™ to control or prevent external parasites in a variety of cultured fish.  Mark P. Gaikowski and Jeff J. Rach. 

Study protocol for a compassionate aquaculture Investigational New Animal Drug Exemption (INAD #10-023).  Clinical field trials to determine the efficacy of Perox-Aid™ to control or prevent mortalities associated with saprolegniasis in a variety of cultured fish and their eggs.  Mark P. Gaikowski and Jeff J. Rach. 

Pivotal dose-confirmation studies to evaluate the efficacy of formalin to control mortalities associated with Saprolegniasis infections on channel catfish.  Jeff J. Rach. 

Efficacy of hydrogen peroxide to control mortalities associated with Saprolegniasis infections on channel catfish.  Jeff J. Rach. 

Pivotal dose-confirmation studies to evaluate the efficacy of potassium permanganate to control mortalities associated with Saprolegniasis infections on channel catfish.  Jeff J. Rach. 

Pivotal dose-confirmation studies to evaluate the efficacy of hydrogen peroxide to control mortalities associated with Saprolegniasis infections on channel catfish.  Jeff J. Rach. 

Efficacy of chloramine-t to control mortality associated with external columnaris on walleye fingerlings (Stizostedion vitreum). Jeff J. Rach. 

Efficacy of formalin to control mortalities associated with Saprolegniasis infections on channel catfish.  Jeff J. Rach. 

Efficacy of hydrogen peroxide to control mortalities associated with Saprolegniasis infections on channel catfish.  Jeff J. Rach. 

Efficacy of hydrogen peroxide to control mortalities associated with Saprolegniasis infections on rainbow trout.  Jeff J. Rach.

Pivotal dose-titration studies to evaluate the efficacy of hydrogen peroxide to control mortalities associated with external flavobacter infections on cultured fish at selected fish hatcheries.  Jeff J. Rach.

Pivotal Studies to Evaluate the Efficacy of Hydrogen Peroxide to Control Parasite Infections on Cultured Fish at Selected Fish Hatcheries.  Jeff J. Rach. 

Efficacy of oxytetracycline-hydrochloride bath immersion to control mortality associated with external flavobacter infections on cool- and warmwater fish cultured at hatcheries.  Jeff J. Rach. 

Environmental Assessment

Validation of a chemical dilution model for hatchery effluent using rhodamine WT and chloramine-T at the Upper Midwest Environmental Sciences Center.  Mark P. Gaikowski.

Chronic toxicity of hydrogen peroxide to the Cladocera, Daphnia magna, in a flow through continuous exposure system.  Jeff R. Meinertz. 

Human Food Safety

Depletion of Aquaflor® (50% Type A Medicated Article), florfenicol - SCH25298, from the edible fillet of tilapia (Oreochromis niloticus x O. niloticus and O. niloticus x O. aureus) following administration in feed.  Mark P. Gaikowski. 

Evaluation of a proposed determinative method for determining concentrations of isoeugenol in fillet tissue from cold, cool, and warm water fish species.  Jeff R. Meinertz. 

Evaluation of a method to determine concentrations of isoeugenol in the edible fillet tissue from rainbow trout.  Jeff R. Meinertz. 

Oxytetracycline residue depletion from the skin-on fillet tissue of coho salmon treated at water temperatures below 9ºC.  Jeff R. Meinertz. 

Development of a regulatory method for p-TSA in the edible fillet tissue of channel catfish and walleye; evaluation of readiness to perform.  Jeff R. Meinertz. 

Depletion of para-Toluenesulfonamide from the edible fillet tissue of rainbow trout after exposure to chloramine-t.  Jeff R. Meinertz.

Depletion of para-Toluenesulfonamide from the edible fillet tissue of hybrid striped bass (Morone saxatilis x M. chrysops) after exposure to chloramine-t.  Jeff R. Meinertz. 

Depletion of para-Toluenesulfonamide from the edible fillet tissue of yellow perch after exposure to chloramine-t.  Jeff R. Meinertz. 

Development of a regulatory method for p-TSA in the edible fillet tissue of rainbow trout; evaluation of readiness to perform and bridging with a previously reported method to determine chloramine-t and p-TSA concentrations in rainbow trout tissue.  Jeff R. Meinertz. 

Validation of an analytical method to determine concentrations of para-Toluenesulfonamide in the edible fillet tissue of cold, cool, and warm water fish.  Jeff R. Meinertz. 

Evaluation of a simple analytical procedure to replace LC for monitoring treatment concentrations of chloramine-t on fish culture facilities.  Jeff R. Meinertz. 

Depletion of isoeugenol from the fillet tissue of yellow perch (Perca flavescens) after exposure to AQUI-S™.  Jeff R. Meinertz. 

Isoeugenol total residue depletion in the edible fillet tissue of rainbow trout.  Jeff R. Meinertz. 

Target Animal Safety

Target animal safety of TM-100F Type A medicated article (220 g oxytetracycline/kg) administered in feed to hybrid striped bass Morone chrysops x M. saxatilis, walleye Stizostedion vitreum, and yellow perch Perca flavescens.  Mark P. Gaikowski.

Target animal safety of Aquaflor® (50% Type A Medicated Article), Florfenicol - SCH25298, administered in feed to channel catfish, Ictalurus punctatus.  Mark P. Gaikowski.

Target animal safety of chloramine-t to cool- and warmwater fish.  Mark P. Gaikowski.

Toxicity assessment of hydrogen peroxide to cold-, cool-, and warmwater fish.  Mark P. Gaikowski.

Hydrogen Peroxide treatment toxicity to rainbow trout eggs.  Mark P. Gaikowski.

Safety of hydrogen peroxide to paddlefish Polyodon spathula and rainbow trout Oncorhynchus mykiss eggs.  Jeff J. Rach.